Part:BBa_K245051:Design

GyrB-SG-SG-CutA1

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 428
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 100
  Illegal AgeI site found at 238
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The part is inserted in BB-NIC-II-HisN vector (BBa_K245005) and can be assembled with other parts according to assembly standard 37. Composite parts were assembled (iGEM 2009, team Slovenia) by cutting front inserts with EcoRI and BspEI and front vectors with EcoRI and NgoMIV, thus forming RFC 37 Scar Sequence (BBa_K245009) beween separate parts.

Source

Composed from GyrB-SG and CutA1 basic parts according to assembly standard 37.